Donna Vekic upsets Wimbledon champion Elena Rybakina at German Open
The second seed, 24, lost 6-7 (1-7) 6-3 6-4 to Croat Donna Vekic in Berlin.
The Kazakh world three had beaten Russian Polina Kudermetova the previous night, with matches delayed because of rain.
Rybakina was among seven of the world's top-10 ranked women competing at the Wimbledon warm-up event.
Vekic faced just two break points against the Kazakh, who pulled out of the French Open before her third-round match earlier this month because of illness.
The Australian Open quarter-finalist saved them both and went on to end Rybakina's eight-match winning streak on grass.
Rybakina's Wimbledon final opponent Ons Jabeur also began her grass-court season with defeat in Germany on Tuesday.
Elsewhere, French third seed Caroline Garcia secured her place in the quarter-finals with a 6-3 6-2 win over Australian qualifier Jaimee Fourlis.
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